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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be noticed in several tumors, which leads to chromosome instability, and thus could lead to tumorigenesis. This protein was discovered to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential position in selling mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are explained. Two added splice variants ended up described but in depth nucleotide sequence details isn't nonetheless out there.
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Blocking peptides are peptides that bind precisely on the target antibody and block antibody binding. These peptide normally includes the epitope regarded from the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This guideline outlines even further achievable optimization of mobile and immunoassay parameters to be sure the very best final results are...
Antibodies bound to the blocking peptide not bind to your epitope on the goal protein. By evaluating the staining within the blocked antibody compared to the antibody by yourself, you can see which staining is unique.
Goal Data Cyclin E belongs into the extremely conserved cyclin loved ones, whose users are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation designs which lead on the temporal coordination of every mitotic function. Cyclin E kinds a fancy with and features like a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates for the G1-S period boundary which is degraded as cells progress by way of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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